Using the BIOREADER® 7000 -V you may analyze enzymatic Elispot in 96 well plates as well as other applications liek Plaque/FOCI in different multiwell plates.

With common fixed focal length objectives also other plate types in full field-of-view are possible: 6- to 1536 well plates, Terasaki plates, upt to 100 mm Petri dishes and cell culture bottles (like T-Flask).

And they provide a resolution of: 100 – 2000 µm for FOCI, 100 – 5000 µm for Plaques or 50 – 2000 µm for cell clones.

The automatic objective changer and camera focusing are software-controlled.

This Bioreader® is able to quantify visible stain. For instant CV, DAB (3,3′-Diaminobenzidine), Substrates TMB,PCIP/NBT, AEC

Model variants:

Basic: 12 MPixel camera, 1 fixed focal length objective, for example for 96 well plates (with high resolution), as well as 48 and 24 well plates (with lower resolution).

Alpha: 5 MPixel camera, 2-times objective changer, for example for 96 and 24well plates with high resolution (with high resolution).

Beta: 5 MPixel camera, 3-times objective changer, for example for 96, 24 and 6 well plates with high resolution (with high resolution).

Gamma: 5 MPixel camera, 4-times objective changer, for example for 96, 24 6 well plates and 50 mm cell culture plates (with high resolution).

Tests of antiviral substances

This Bioreader® and EazyReader® support testing and development of antiviral substances.

Example#1: The automatic analyses of Plaque reductions assay.

This assay applies diluted doses of a -possibly- antiviral drug into multiwell plates. The Bioreader automatically observes its capability to suppress the formation of plaques (-holes-) in the bacterial lawn.

The ‘Plaque reductions neutralization test’ (PRNT) is a variation of this test. It is an attempt to find an antibody that neutralizes a specific virus. This is a very valuable tool in antiviral vaccine research.

Examplel#2: The automatic evaluation of the Yield reduction assay

In several steps of operation cells are infected in presence of variable doses of a possibly antiviral drug substance.

Thereafter the virus titer is determined in the liquid by use of the plaque or TCID50 assay with of the Bioreader®.

The titer is calculated as the quotient of the positive cells/all cells, taking in account the virus dilution factor and the volume of the inoculum.

Features

• Patented dual telecentric illumination.
• Front loader with automatic door.
• Lens changer for high definition lenses with fix focal length.
• Automatic focus, illumination and lens changer are measure protocol controlled (GxP locked).
• High resolution camera with 5 MPixel (up to 42 MPixel available).
• Incl. computer and monitor.
• Excel/Word/PowerPoint and LIMS export capabilities.
• Interactive content specific video training program.
• Suitable for research purpose.
• For ‘phantom spot suppression’, better spot separation and cytokine quantification.
• Optional automation with plate ‘feeding system’.
• DQ/IQ/OQ/PQ documentation possible.
• 12 months warranty.
• EC declaration of conformity and EMC certificate.