CellVoyager CV8000 High-Content Screening System
The CV8000 features a cell incubator with an improved airtight design that facilitates the observation of cell behavior over long periods of time. In addition, the CV8000 comes with CellPathfinder, a new program that can analyze images of unlabeled cells and 3D images of samples. With these features, the CV8000 improves the efficiency of drug discovery research and biomedical research on leading-edge subjects such as iPS and ES cells.
CV8000 System Highlights
| Excitation laser wavelength | 405nm、445nm、488nm、561nm、640nm |
| Illumination source | Laser |
| Objective lens | 2x to 60x (Dry, Phase contrast, Water immersion, Long working distance) |
| Camera | High-sensitivity sCMOS camera (up to 4 units) |
| Autofocus | Laser-based mode, image-based mode |
| Software | CellPathfinder、CellLibrarian |
Introduction
Within the drug development market, demands on high content analysis systems for drug efficacy evaluation are increasing in accordance with the needs for cell-based assay and phenotypic screening. In order to increase screening efficiency, devices with higher speeds (higher throughput) are required.
On the other hand, in order to bridge the “valley of death” of the drug development process, the quality of screening hits must be increased.
This requires the construction of more complex evaluation systems that utilize multifaceted parameters via 3D cultivation systems, live-cell imaging and higher detail image analysis.
In current drug development research, determining how to implement throughput screening and complex evaluation system screening in parallel is an important issue.
Solution
The CellVoyager CV8000 is a high-end, high content analysis system that solves this contradictory screening challenge.
Through the combination of a proprietary Yokogawa high speed confocal scanner, water immersion lens, up to four high field-of-vision cameras, a microscopic stage with cell cultivation environment, and an integrated robotic pipetter, we have realized not only high throughput, high-resolution imaging, but also phenotypic screening via a more complex evaluation system.
In addition, our specialized analysis software, CellPathfinder, uses deep leaning and machine learning to recognize target objects with high accuracy, supporting you from image analysis to results display using graphs.
The Yokogawa advantage
- Confocal scaner unit
- Live/kinetic experiment compatible
- High throughput
- Reliable, proven technology
Voyage to unknown worlds
Real time confocal, label-free imaging
Long-term livecell imaging
Stage incubator included as standard. Realization of non-stop, long-duration observation (3 days +) via humidity, temperature and CO2 control.
Left：Before incubation Right：After 68 hours incubation
Kinetic assay
Drug addition during imaging is made possible by an integrated robotic pipetter with disposable tips.
Ideal for kinetic experiments involving the observation of high speed phenomena.
Left：Before incubation Right：After stimulation
Organoid / Spheroid
Yokogawa’ s spinning disk confocal technology excels in imaging of samples with depth, such as 3D culture samples where clear and quick imaging is difficult, allowing for evaluation close to in-vivo quality.
Left：Original image Right：Recognition image
Label-free analysis
Recognition and analysis can be performed by taking bright field images from several Z positions and creating a CE bright field image using the included CellPathfinder analysis software. Analysis accuracy is further enhanced via the new Deep Learning option.
Left：CE Bright Field Right：Cell Recognition image