3D CELL EXPLORER-fluo
Multimodal complete solution: 4D live cell imaging & fluorescence
3D Cell Explorer-fluo
- Combine fully label-free imaging with correlative epifluorescence to cover a broad array of live cell imaging requirements for your lab, department, or institute
- Rediscover your living cells through multiplexing: Nanolive datasets contain the simultaneous acquisition of several biological features and organelles – therefore capturing various pathways of cell biology in real-time
- Extend Live Cell Imaging: image your live cells as long as you need. Limit cell damages caused by fluorescent markers, bleaching and phototoxicity
- Visualize the invisible: the 3D Cell Explorer-fluo allows you to visualize what would be too small to be captured label-free, such as: proteins, ions (e.g. calcium), viruses, etc
Video: Linking changes in cell morphology to adenovirus infection
Applications
A selected overview of Nanolive’s top applications.
Neuroscience
Visualizing mitochondrial trafficking in neurons
Stem cells
Visualizing stress fibers in mesenchymal stem cells
Immuno-oncology
Human bone osteosarcoma epithelial cells (U2OS) cells expressing Histone 2B-GFP and LAMP1-RFP
Fully integrated cutting-edge digital analytical solutions
Get higher significance earlier and faster with our panel of application specific, push-button digital assays. Retroactive analysis is also possible at any time.
Cytotoxicity
LIVE Cytotoxicity Assay
Profile cell health and death, and distinguish between living, apoptotic, and necrotic cells label-free.
Immuno-oncology
LIVE T Cell Assay
Measure and characterize simultaneously how live T cells find, bind, stress, and serial kill their targets.
Metabolism
Smart Lipid Droplet Assay
Measure lipid droplet characteristics at population, cellular, and individual droplet levels.
Disruptive Technology
The combination of holography and rotational scanning makes Nanolive imaging a revolutionary technology
Nanolive’s technology is unique because it works label-free and reports the 3D refractive index distribution of the cell. The extremely low light power that generates the holograms allows for a total absence of phototoxicity which leads to excellent time resolution.
Top Image: 10h time-lapse of mESC undergoing mitosis (Refractive Index and 3D visualization). Bottom image: Organelles which are visible label-free with Nanolive technology: a. mitochondria; b. plasma membrane; c. actin fibers; d. lipid droplets; e. lysosomes; f. nuclear envelope, nucleus & nucleoli.