Combine the Best of two Worlds
COMPLETE 3D SOLUTION
Combine high quality tomographic data with fluorescent markers
MULTIPLEXING
Explore multiple cell compartments
EXTENDED LIVE CELL IMAGING
Limit cell damages caused by fluorescent markers, bleaching and phototoxicity
Winner of Basel Life MipTec Product-Innovation Award 2017
Available Configurations
DAPI configuration: DAPI + FitC + TritC
Live Cell Imaging configuration: FitC + TritC + Cy5
All-in-one configuration*: DAPI + FitC + TritC / Cy5
*TritC and Cy5 excitation filters are exchangeable on channel 3
Multimodal Complete Solution for 3D Live Cell Exploration
Combine 3D refractive index analysis with a fully integrated 3 channel epifluorescence module to image your live cells as they are and as long as you want. Put chemical information into structural context for new biological insights.
2D section of RI map
Multi-channel epifluorescence
Overlay
3D reconstruction based on RI map
Multiplexing
Transform 2D fluorescence into 3D cell tomography: Identify cell organelles through fluorescence and monitor non-invasively their structures and dynamics in 3D & marker-free.
Co-localization for mitochondria
Co-localization for lipid droplets
Co-localization for lysosomes
Co-localization for plasma membrane
Co-localization for nuclear membrane
Co-localization for nucleus & nucleoli
Extended Live Cell Imaging
Image your live cells as long as you need. Limit cell damages caused by fluorescent markers, bleaching and phototoxicity.
Discover More
Combine high quality RI tomographic data and fluorescence markers
You want to extend your understanding of cell structures and cell mechanisms? Thanks to our 3D tomographic technology you can now have a powerful platform that combines RI information with fluorescence markers.
Go beyond fluorescence limitation
You are frustrated by the limitations of fluorescence markers? You would like to go further? Combine them with Nanolive’s digital staining and push the limits forward.
Achieve specific cell analysis while preserving cell life
Forget about fixation! With the 3D Cell Explorer-fluo you can now observe your cells as they are: alive, 3D and in motion!
Perform infinite live cell imaging
Limit cell damages caused by fluorescence markers, bleaching, and phototoxicity. The 3D Cell Explorer-fluo allows you to transform your chemical markers into physical ones which can be monitored for a limitless amount of time.
| Illumination source | Holotomography (HT): Class 1 laser low power (λ=520 nm, sample exposure 0.2 mW/mm2) Epifluorescence: CoolLED pE-300ultra, High speed switchable <100 μs, Lifetime >20’000 hours each channel |
|---|---|
| Resolution | 3D HT: x,y: 200 nm; z: 400 nm (3D image) Epifluorescence: rx,y: ~ 400 nm (2D image) |
| Field-of-view | HT: 90 × 90 × 30 μm Epifluorescence: 90 × 90 μm |
| Microscope Objective | Dry objective / 60x magnification / NA 0.8 |
| Channels | HT: Up to 7 simultaneous Epifluorescence: DAPI + FitC + TritC | FitC + TritC + Cy5 | DAPI + FitC + TritC / Cy5 |
| Imaging | HT: 3D Epifluorescence: 2D 4D time lapse: (RI + fluo) |
| Time resolution | HT: 0.5 fps 3D RI frame Epifluorescence: 3 fps each channel |
| Camera | USB 3.0 CMOS Sony IMX174 sensor / Quantum Efficiency (typical) 70 % (at 545 nm) / Dark Noise (typical) 6,6 e¯ / Dynamic Range (typical) 73,7 dB |
| Dimensions (width × depth × height in mm) | 3D Cell Explorer-fluo: 380 × 170 × 445 Fluorescence module: 77 × 186 × 162 |
| Weight | 12kg |