IntelliGene^® Human Cytokine CHIP Ver.3.1 is a product which is arrayed and immobilized with approximately 550 cDNA fragments of human identified genes related to cytokine on a slide glass. About 300 bp cDNA region of each gene, which have minimal homology against other genes registered at cDNA database, are spotted on this DNA microarray. Also DNA fragments derived from human housekeeping genes such as β-actin and derived from other species such as lambda DNA are spotted on this product as control spots for hybridization. These DNA fragments are arrayed at the colored area on the slide glass figure. The expression of each gene can be simultaneously analyzed through hybridization of the probes which are prepared by using RNA (total RNA or polyA⁺ RNA) obtained from human cells as a template. Control spots can be used to normalize the signal intensity between fluorescence-labeled probes and to determine the background level. Array list for this product contains Gene name, Cluster ID of UniGene and Accession number of GenBank. The gene name has been determined from UniGene clustering (UniGene Homo sapiens database) based on the DNA sequence.

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