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IntelliGene®E.coli
CHIP Ver.2.0 is a product which is arrayed and
immobilized with DNA fragments of E.coli genes,
prepared from Archive Plasmid containing the ORFs
derived from E.coli K12W3110 strain, on a slide
glass. Approximately 4,150 DNA fragments are arrayed,
which cover about 97% of total ORFs. About 300 bp DNA
region of each gene, having minimal homology against
other genes registered at database, are spotted on this
DNA microarray. Also DNA fragments derived from other
species such as human TFR are spotted on this product as
negative control spots for hybridization. These DNA
fragments are arrayed at the colored area on the slide
glass figure. The expression of each gene can be
simultaneously analyzed through hybridization of the
probes which are prepared by using total RNA obtained
from E.coli cells as a template. Control spots
can be used to determine the background level. Further
detailed information about OFR ID, gene name, and
description in Array list can be obtained from
Escherichia coli WWW HOME PAGE (http://ecoli.aist-nara.ac.jp/).
Detailed gene information
for all of IntelliGene® products are
available to download as Array list(text file) and
GeneID file(text file) for ImaGene software through
TaKaRa website.
Click here!
Further information of each
ORF can be obtained from GenoBase version 2.0 [Escherichia
coli K-12 W3110] (http://ecoli.aist-nara.ac.jp/) of
Nara Institute of Science and Technology.
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Storage
Store in a desiccator,
packed in vessel at room
temperature in the dark.
Keep cool, dry and
protected from light.
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