|
<< Back
TaKaRa IntelliGene®
HS Human Expression CHIP is a microarray immobilized
with approximately 16,600 human genes which are reported
to have more secured expression, based on NCBI Reference
Sequence (RefSeq). The spotted DNA regions are designed
to be lee than 1.5 kb from the 3'-end of RNA transcript,
and about 300 bp length of each gene which have minimal
homology against other genes registered at cDNA
database, are spotted on this DNA microarray.
Accordingly, the obtained analysis data with this
microarray reflects each RNA transcript more correctly.
In addition, the sense strands of cDNA are immobilized
on the slide through covalent bond, at this microarray
utilizes TaKaRa-Hubble Slide Glass which offers high
immobilization efficiency and lower background.
The expression of each gene
can be simultaneously analyzed through hybridization of
the probes which are prepared by using RNA (total RNA or
polyA+ RNA) obtained from mouse cells as a
template. Control spots can be used to normalize the
signal intensity between fluorescence-labeled probes and
to determine the background level.
Detailed gene information
for all of IntelliGene® products are
available to download as self-extracting text file and
GeneID file for ImaGene software through TaKaRa website.
Click here!
Array list for this product
contains Gene name, RefSeq ID, Locus ID of LocusLink,
and the term of Gene Ontology. Each Gene Name is the
same this is shown in RefSeq of NCBI.
.............................................................................................................................................................................................
Storage
Store in a
desiccator, packed in vessel at room
temperature in the dark.Keep cool, dry
and protected from light.
Product
version and number of genes spotted may
be subject to upgrade. Please visit the
TaKaRa website at www.takara-bio.com for
the latest product information or to
download a complete list of the genes
spotted on each IntelliGene® microarray.
.............................................................................................................................................................................................
Control Spots
-
cDNA fragments
derived from
human
(as housekeeping
genes)
|
Cnt 1 : |
actin,
alpha 2,
smooth
muscle,
aorta
(ACTA2) |
|
Cnt 2 : |
actin,
beta (ATCB) |
|
Cnt 3 : |
ATP
synthase,
H+
transporting,
mitochondrial
F0
complex
(ATP5F1) |
|
Cnt 4 : |
general
transciption
factor
IIB
(GTF2B) |
|
Cnt 5 : |
glyceraldehyde-3-phosphate
dehydrogenase
(GAPD) |
|
Cnt 6 : |
hexokinase
1(HK1),
transcript
variant
4 |
|
Cnt 7 : |
histone
2, H2be
(HIST2BE) |
|
Cnt 8 : |
ribosomal
protein
S27a
(RPS27A) |
|
Cnt 9 : |
ribosomal
protein
S5(PRS5) |
|
Cnt 10 : |
transferrin
receptor
(p90,
CD71)(TFRC) |
|
Cnt 11 : |
tubulin,
alpha 2
(TUBA2),
transcript
variant
2
|
-
DNA fragments
derived from
other species
|
Cnt 12 : |
E.coli
L-arabinose_isomerase_(EC_5.3.1.4) |
|
Cnt 13 : |
synechocystis
sp.
phoyosystem_II_D1_protein |
|
Cnt 14 : |
lambda-A |
.............................................................................................................................................................................................
Usage
Refer to
the attached IntelliGene™
Users Manual. In case of detection by
Affymetrix Array Scanner, load the
microarray onto the scanner to face the
label side. Set the parameters for
reading positions as follows.
|
Scan Offset Y (mm)
|
Scan Size Y (mm)
|
|
IntelliGene™ HS
Human Expression CHIP |
15 |
50 |
............................................................................................................................................................................................. |