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IntelliGene® Rat Toxicology
CHIP Ver.1.0 is a product which is arrayed and
immobilized with approximately 390 cDNA fragments of rat
(Rattus norvegicus) identified genes related with
the response to stress and toxicity on a slide glass.
About 300 bp cDNA region of each gene, which have
minimal homology against other genes registered at cDNA
database, are spotted on this DNA microarray. Also DNA
fragments derived from rat housekeeping genes such as β-actin
and derived from other species such as lambda DNA are
spotted on this product as control spots for
hybridization. These DNA fragments are arrayed at the
colored area on the slide glass figure. The expression
of each gene can be simultaneously analyzed through
hybridization of the probes which are prepared by using
RNA (total RNA or polyA+ RNA) obtained from
rat cells as a template. Control spots can be used to
normalize the signal intensity between
fluorescence-labeled probes and to determine the
background level. Array list for this product contains
Gene name, Cluster ID of UniGene and Accession number of
GenBank. The gene name has been determined from UniGene
clustering (UniGene Rattus norvegicus database)
based on the DNA sequence.
Detailed gene information
for all of IntelliGene® products are available to
download as self-extracting text file and GeneID file
for ImaGene software through TaKaRa website.
Click here!
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Storage
Store in a
desiccator, packed in vessel at room
temperature in the dark.
Keep cool, dry and protected from light.
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