Products >> Genomics >> Ready-to-Use Prespotted cDNA Microarrays >> IntelliGene® II Mouse CHIP
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IntelliGene® II Mouse CHIP is a product which is arrayed and immobilized with approximately 4280 cDNA fragments of mouse identified genes on a slide glass. Approximately 300 bp cDNA region of each gene, which have minimal homology against other genes registered at cDNA database, are spotted on this DNA microarray. Also DNA fragments derived from mouse housekeeping genes such as β-actin and derived from other species such as lambda DNA are spotted on this products as control spots for hybridization. These DNA fragments are arrayed at the colored area on the slide glass figure.

The expression of each gene can be simultaneously analyzed through hybridization of the probes which are prepared by using RNA (total RNA or polyA+ RNA) obtained from mouse cells as a template. Control spots can be used to normalize the signal intensity between fluorescence-labeled probes and to determine the background level.

Detailed gene information for all of IntelliGene® products are available to download as self-extracting text file and GeneID file for ImaGene software through TaKaRa website.

Click here!

Array list for this product contains Gene name, Cluster ID of UniGene and Accession number of GenBank. The gene name has been determined from UniGene clustering (UniGene Mouse Sequences Collection) based on the DNA sequence.

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Storage

Store in a desiccator, packed in vessel at room temperature in the dark.
Keep cool, dry and protected from light.

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Control Spots

  • cDNA fragments derived from mouse
    (as housekeeping genes)
    Cnt 1 : actin, beta 12 spots
    Cnt 2 : ornithine decarboxylase 12 spots
    Cnt 3 : myosin Ib 12 spots
    Cnt 4 : HPRT (hypoxanthine guanine phosphoribosyl transferase) 12 spots
    Cnt 5 : tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein 12 spots
    Cnt 6 : ribosomal protein S18 12 spots
    Cnt 7 : GAPDH (glyceraldehyde-3-phosphate dehydrogenase) 12 spots
    Cnt 8 : transferrin receptor (Trfr) 12 spots
    Cnt 9 : transferrin receptor2 (Trfr2) 12 spots
    Cnt 10 : Sdf4 (stromal cell derived factor 4)(Ca2+ binding protein) 12 spots
  • DNA fragments derived from other species
    Cnt 11 : lambda-A 12 spots
    Cnt 12 : lambda-B 12 spots
    Cnt 13 : pUC19 12 spots
    Cnt 14 : Arabidopsis
    chlorophyll ab binding protein    		 12 spots

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