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Takara' s
IntelliGene® series consists of DNA
microarray sets which are arrayed at high density and
immobilized with DNA fragments derived from a large
number of genes on glass slides and allow quick and
simple analysis of the expression of a large number of
genes at the same time.
In principle, IntelliGene® DNA microarrays
for eukaryote are spotted with cDNA regions including
3'-UTR without Alu sequence, and the ones for prokaryote
are spotted with full-length or 3'region of ORFs. It
enables to detect nucleic acids complementary to each
DNA fragment on the slide by the principle of
hybridization. For instance, two kinds of RNA samples
derived from cells propagated under different conditions
are labeled with different fluorescent dyes (Cy™3
or Cy™5) and subjected to competitive
hybridization on a single DNA microarray. The
fluorescent intensity of each dye at each spot is
determined by the microarray scanner and a difference in
gene expression between the two samples as an increase
or decrease of one dye relative to the other.
Information about the DNAs spotted on Takara's DNA
microarray products can be downloaded from our website.
URL:
http://www.takara-bio.co.jp/dnachip_e/dnachip_download_e.htm
At the website, we provide an Array list (text file) and
a GeneID file (text file). The Array list is a list of
genes arrayed on the DNA microarray and their position
on the slide. The accession number (database such as
GenBank, UniGene) is also a provided. The GeneID file is
used in data analysis with the analysis software, such
as ImaGene™ (BioDiscovery, Inc.), and links
the experimental results to the gene name.
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Analysis Example
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Intelligene® Cyano CHIP Ver. 2.0 |
Intelligene® Rat Toxicology CHIP
Ver. 1.0 |
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