Products >> Proteomics >> Protein Expression >> pCold™ DNA - Cold Shock Expression System
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Description

Cold-shock expression vectors, pCold™ DNA, are designed to perform efficient protein expression utilizing promoter derived from cspA gene, which is one of the cold-shock genes. At the downstream of the cspA promoter, lac operator is inserted so that the expression is strictly controlled. In addition, 5′ untranslated region (5′ UTR), translation enhancing element (TEE), His-Tag sequence, Factor Xa cleavage site, and multicloning site(MCS) are located at the downstream of the cspA promoter. As this product utilizes the promoter derived from E. coli, most E. coli strains can be utilized as an expression host.
There are four kinds of pCold™ vectors, whose arrangements vary in the existence of TEE, His-Tag sequence and Factor Xa clevage site.

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Storage

-20°C

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Features

	High Yield Recombinant Protein: Up to 60%, at maximum, of expressed intracellular protein is target protein.
	Soluble expression level is increased: Proteins that are insoluble in conventional expression systems can be expressed in soluble form.
	Wide Range of E. coli Hosts: Highly suitable for most E. coli strains.
	Compatible with Chaperone Plasmids Vectors: When used in conjuction with one of Takara's Chaperone Plasmids, the amount of recoverable soluble protein can be further increased.
	Radioisotope Labeling: Up to 90%, at maximum, of newly expressed cellular protein is labeled target protein.

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Vector Map

 

	TEE	His Tag	Factor Xa Cleavage Site
pCold™ I DNA	Ο	Ο	Ο
pCold™ II DNA	Ο	Ο	×
pCold™ III DNA	Ο	×	×
pCold™ IV DNA	×	×	×

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GenBank

	Accession No.
pCold I	AB186388
pCold II	AB186389
pCold III	AB186390
pCold IV	AB186391

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Form

10 mM Tris-HCl (pH8.0), 1 mM EDTA

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Chain length

pCold™ I DNA : 4,407 bp
pCold™ II DNA : 4,392 bp
pCold™ III DNA : 4,377 bp
pCold™ IV DNA : 4,359 bp

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Purity

	Contains over 70% double-stranded covalently closed circular DNA (RF I) by agarose gel electrophoresis.
	Confirmed to maintain cloning sites by dideoxy sequencing method.
	Confirmed to be cleaved at a single site by restriction enzymes Nde I, Sac I, Kpn I, Xho I,BamH I, EcoR I, Hind III, Sal I, Pst I and Xba I.

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Application

Protein Expression utilizing the promoter of cold shock gene (cspA).

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