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This product is a solution to dissolve DNA when producing DNA microarrays with TaKaRa-Hubble Slide™ Glass (Cat. #TX720) by immobilizing DNA through covalent bond. The DNA fixation efficiency can be improved by preparing DNA in this solution. Since this solution is supplied at 4 × concentration, use at 1 × concentration.

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Storage

4°C

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Usage

Dissolve amino-modified DNA in sterilized distilled water, and add Solution I (4 ×) in one-third volume of the DNA solution. After mixing well, transfer the prepared DNA solution to a microplate and set it on Affymetrix 417/427 Arrayer.

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Storage of DNA solution

DNA solution prepared with Solution I should be stored at –20°C.
It can be stored at 4°C for 2-3 days.

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Note

This product is a DNA dissolving solution developed for exclusive use with TaKaRa-Hubble Slide™ Glass. DNA may not be immobilized when it is used with other slide glasses.