Products >> Proteomics >>
IEF System
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Dedicated 2-D Electrophoresis Solutions

SCIE-PLAS now provide the first of a series of innovative solutions for scientists who prefer the latest advances in IPG (immobilised pH gradient) strip technology to more traditional capillary gels as the first step of 2-D electrophoresis. Our recently improved flat-bed isoelectric focusing system (IEF-SYS) allows 1st dimension 2-D electrophoresis to be performed with multiple IPG strips within the same tank, while 2nd dimension SDS-PAGE is carried out in our existing TV100 and 400 Mini- and Maxi-gel formats (TV100YK orTV400YK).

Mini format:

TV100YK-2DIEF-SYS

Dedicated 2-D electrophoresis system comprising the IEF-SYS flat-bed isoelectric focusing unit and 10 x 10cm TV100YK mini-gel format for 2nd dimension electrophoresis.

 

Maxi format:

TV400YK-2DIEF-SYS 

Dedicated 2-D electrophoresis system comprising the IEF-SYS flat-bed isoelectric focusing unit and 20.5 x 20cm TV100YK maxi-gel format for 2nd dimension electrophoresis.

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TV100 and 400YK-2D-IEF-SYS Design Benefits  

For 1st dimension IEF: -
 

A recently redesigned IEF-SYS unit where multiple IPG strips, up to 25cm in length, can be run simultaneously on a 27 x 27cm ceramic cooling plate

Positive and negative electrodes within the glass electrode frame that can be adjusted to the exact length of IPG strip for fine-tuning of the voltage gradient

A printed grid allows measurement of the migration rate, whereas supercooling, provided by cooling plate or the optional KELECTRO4 chiller, guarantees high voltage isoelectric focusing

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For 2nd dimension SDS-PAGE: -

A flexible gel system where SDS-PAGE can be performed according to personal preference in existing TV100YK and TV400YK mini- and maxi-gel formats
1mm-thick colour-coded spacers and 1-D combs compatible with the standard thickness of commercially available IPG strips
Guaranteed leak-free notched gel-running module (GRM) and cam-pin casting base
Built-in cooling coil, compatible with the optional K-ELECTR04 chiller or lab water supply, enables electrophoresis to be performed faster and with greater resolution.

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