Quantitative. Use
luciferase activity to measure TF binding
Flexible. Use for in vivo
assays
Rapid. Get results in hours
Stable. Store for up to one
year
Safe. No radioactivity
required
Wide array of carefully optimized reagents designed
to induce transcription factors.
With Panomics Luciferase Reporter Vectors, you can
monitor and even quantify the binding activity of
transcription factors. Each Reporter Vector contains
a cis-acting DNA binding element that is recognized
by a specific TF. Binding at this site results in
the expression of firefly luciferase, an enzyme
capable of catalyzing a powerful bioluminescent
reaction. Light emitted from the chemical reaction
is directly proportional to the amount of expressed
enzyme and thus the binding activity of the targeted
TF.
Like our EMSA gel-shift kits, the Panomics Reporter
Vectiors are ideal for validating the results
obtained using Protein/DNA Arrays. But while EMSA
only allows in vitro verification of those results,
the use of the Luciferase Reporter Vectors allows TF
activity to be monitored in living cells. Don't
settle for answers from the test tube; investigate
TF binding where it happens!
