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GPCR

Kinases

Biomarkers

Assays on Demand

Custom Labelling

HTRF innovations

Experience Cisbio's latest developments


Unable to find an assay that suits your need or you want to convert your ELISA into a homogeneous method. Through Assays on demand, Cisbio Bioassays gives you a list of its latest developments for the Drug Discovery field. To see the extent of our expertise and know-how, and to find a solution that will fulfil your specific needs, see our service offerings.

These assays on request are sold frozen and available in 1,000 and 20,000 test volumes.

 

 

SIRT1 NEW FORMAT!

Format: acetylated d2-labeled substrate recognized by a Eu(K)-labeled antibody. Signal is directly linked to enzyme activity. Maximum signal is obtained without enzyme and decreases as the enzyme removes the acetyl group from the substrate.

Applications: High Throughput SIRT1 inhibitor or activator Screening.

Features :
Incubation time (enzymatic step): 30 min
Enzyme titration EC50: 22 mU/well
Z' at EC50 > 0.7

Aldosterone

Format: competitive immunoassay

Application: quantitative measurement of aldosterone and aldosterone synthase activity assessment

Features :
Incubation time: 1 hour at RT
Detection limit: 24 pg/mL (initial)
Working range: 24 pg/mL to 17 700 pg/mL
EC50: 453 pg/mL
Cross reactivity:
- Corticosterone: 0.05%
- 11-deoxycorticosterone: 0.0017%

Triiodothyronine

Format: competitive immunoassay

Application: quantitative measurement of T3

Features:
Incubation time: 1 hour at RT
Detection limit: 24 pg/mL (initial)
Working range: 24 pg/mL to 6 000 pg/mL
EC50: 464 pg/mL
Cross reactivity:
- T2: 0.45%
- T4: 1%

IL-2

Format: sandwich immunoassay

Application: quantitative determination of IL-2

Features:
Incubation time: 2 hours at RT
Detection limit: 39 pg/mL (initial concentration)
Linear range: 39 to 5000 pg/mL

LTB4

Format: competitive immunoassay

Application: quantitative measurement of LTB4 in buffer solution or cell supernatants

Features:
Incubation time: 2 hours at RT
Detection limit: 49 pg/mL
Linear range: 228 to 6180 pg/mL
EC50: 1069 pg/mL
Cross reactivity:
- 6-trans 12-epi LTB4: 0.19%
- 6-trans LTB4: 5.5%

Glucagon

Format: competitive immunoassay

Application: quantitative measurement of Glucagon

Features:
Incubation time: 3 hours at RT
Detection limit: 50 pM (final)
Working range: 62 to 555 pM final
EC50: 0.23 nM final

Testosterone

Format: competitive immunoassay

Application: measurement and quantification of Testosterone

Features:
Incubation time: 1H at RT
S/B: 13
Detection limit: 0.1 nM initial
Working range: 0.1 to 10 nM
EC50: 0.84 nM

Mouse IL17

Format: sandwich immunoassay

Application: quantitative determination of mouse IL17

Features:
Incubation Time: 2H at RT
Detection limit : 10pg/ml (diluent) and 20pg/ml (culture medium)
Linear range:15.5 pg/ml to 2000 pg/ml

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