PrimerDesign Reverse Transcription (RT) Kits Getting
Better and Bigger
New and improved high quality reverse transcription
kits
High efficiency Reverse Transcription (RT) ensures
the ability to detect rare transcripts and obtain
the strongest possible real-time PCR signals. A
complete solution, the PrimerDesign Reverse
Transcription kits contain all the reagents
necessary for the RT step. Although PrimerDesign
recommends Oligo-dT priming for most applications, a
random nonamer primer is included in the kit in case
this is your preferred strategy.
Our new Reverse Transcriptase enzyme is especially
modified to work at high temperatures. Higher
reaction temperatures reduce the RNA secondary
structure (tangling) and allow the production of
longer transcripts and make for faster reactions.
The enzyme also has an increased affinity for
primer-template complexes which allow for efficient
transcription of very low amounts of RNA.
Two Step RT Kits
Features :
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New modified MMLV enzyme which can operate
at higher temperatures |
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10 fold increase in affinity for RNA/primer
duplexes |
Benefits :
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Less secondary structure in the RNA at high
temperatures. |
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Improved RT efficiency Higher yields of cDNA. |
Precision qScript reverse transcription kit
(Catalogue No: RT-qScript50)
Designed for research customers. Complete 50
reaction RT kit with qScript enzyme. Suggest range
2ng-2ug RNA, 50 Reactions
Precision picoScript reverse transcription kit
(Catalogue No: RT-picoScript50)
Designed for research customers. Complete 50
reaction RT kit with picoScript enzyme for low yield
RNA experiments. Suggest range 3pg-3ng RNA, 50
Reactions
Precision OneStepTM
qRTPCR MasterMix
Benefits :
Simplicity
The Precision OneStep™ qRTPCR MasterMix contains all
of the required components for a perfect one step
realtime PCR analysis in a single reaction mix. The
operator simply adds RNA and a primer/probe mix for
a complete, closed tube, one step RNA to Ct
reaction.
qScript is a modified thermostable MMLV enzyme with
an optimum working temperature of 55°C and a higher
affinity for primer duplex initiation sites. This
increases the rate of processing and the sensitivity
of detection. The result is an RT step that is
completed in less than 10 minutes and is sensitive
in a linear range across the whole biological scope
from very high (>1X109 copies) to very low (<10
copies) RNA copy numbers. An RNAse inhibitor is
included to protect the sample from possible
degradation. The amplification step components are
based on our high quality Precision MasterMix
product.
Enormous Cost Saving
A one step qRTPCR reaction saves both time and
money. Technician labour/bench time requirements to
run the tests are greatly reduced. In addition, the
need for a separate reverse transcription kit is
eliminated which reduces costs dramatically.
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