Apply the Science of ART: Time Domain Optical Imaging

ART’s Optix MX2 is a high performance optical imaging device designed to characterize, quantify and visualize cellular and molecular events in vivo using fluorescent probes. Based on a proprietary time domain (TD) approach, the Optix MX2 technology can be applied to further understand the mechanism of disease and evaluate the effects of therapy and disease progression.

In TD optical imaging, as opposed to Continuous Wave (CW) fluorescence systems that capture diffused light, short pulses of light driven by pulsed laser diodes are used to illuminate the organism under study and excite fluorescent molecules. Time-of-flight distribution is determined, enabling depth and concentration to be uncoupled. In addition, a fluorescence lifetime is derived, giving an important parameter in the measurement of tissue environment and allowing the distinction between different fluorescent sources.

About fluorescence lifetime

A unique characteristic of ART’s time domain (TD) technology — nanosecond temporal point resolution — enables researchers to acquire in vivo fluorescence lifetime measurements to differentiate fluorophores with similar spectral profiles. In addition, by using probes, researchers can identify microenvironmental changes in pH, oxygen level, temperature and other factors known to affect fluorescence lifetime.

Uses for fluorescence identification with lifetime gating: