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Products >> Next Generation Sequencing (NGS) >> Library Preparation
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KAPA Library Preparation Kits provide all of the enzymes and reaction buffers required for constructing Illumina libraries from fragmented dsDNA and include the following modules:

	End Repair
	A-Tailing
	Ligation
	Amplification

Reaction buffers are supplied in convenient, concentrated master mix formats comprising all of the required reaction components except oligonucleotide adaptors or PCR primers.

KAPA Library Preparation Kits are supplied with either a standard PCR library amplification module or a real-time PCR amplification module.

KAPA Library Preparation Kits contain the following reaction modules:

	End Repair The End Repair module produces blunt-ended, 5’-phosphorylated DNA fragments. The module includes End Repair Enzyme Mix (T4 DNA Polymerase, DNA Pol I, T4 Polynucleotide Kinase) and 10X End Repair Buffer with dNTPs.
	A-Tailing The A-Tailing module adds dAMP to the 3’-ends of the dsDNA fragments. The module includes A-Tailing Enzyme (Klenow exo-) and 10X A-Tailing Buffer.
	Adaptor Ligation The Ligation module ligates dsDNA adaptors with 3’-dTMP overhangs to library fragments. The module includes DNA Ligase and 5X Ligation Buffer.
	Amplification KAPA Library Preparation Kits are supplied with either a standard PCR library amplification module (KAPA Library Amplification Kit) or a real-time PCR amplification module (KAPA Real-Time PCR Library Amplification Kit). Both modules are supplied as a 2X master mix and contain the novel KAPA HiFi DNA Polymerase, engineered for high fidelity and processivity and capable of balanced amplification of complex library DNA. KAPA Real-Time PCR Library Amplification Kit also includes SYBR® Green I dye and four fluorescent standards to allow for the real-time monitoring of library amplification.

Higher yields of adaptor-ligated library molecules

Sheared genomic DNA from three organisms (S. aureus, E. coli, or M. tuberculosis) was prepared in bulk by nebulization and 1 μg of identical starting material was used for each library. Libraries were constructed using the KAPA Library Preparation Kit and recommended protocol (green; 9 libraries), or using the Illumina TruSeq™ DNA Sample Prep Kit and Low-Throughput Protocol (orange; 15 libraries). Libraries were quantified by qPCR before size selection using the KAPA Library Quantification Kit according to the recommended protocol. The estimated percentage of starting material that was converted to useful, adaptor-ligated (PCR-amplifiable) library molecules is provided. The KAPA Library Preparation Kit and protocol produced ~25-fold more adaptor-ligated library fragments from the same amount of starting material than did the standard Illumina TruSeq™ DNA Sample Prep Kit.

Ordering information:-

KAPA Library Preparation Kit with Standard PCR Library Amplification
10 reactions – KK8200
50 reactions – KK8201

KAPA Library Preparation Kit with Real-Time PCR Library Amplification
10 reactions – KK8220
50 reactions – KK8221

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