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KAPA Universal Ladder

Product Description

The KAPA Universal Ladder Kit is designed for determining the approximate size and quantity of double-stranded DNA on agarose gel. KAPA Universal Ladder kits contain eighteen DNA fragments (in base pairs): 100, 150, 200, 300, 400, 500, 600, 800, 1000, 1200, 1600, 2000, 3000, 4000, 5000, 6000, 8000, and 10000. The KAPA Universal Ladder contains four reference bands (500, 1000, 1600, and 4000) for orientation. Kits are formulated with DNA loading dye for direct loading on agarose gel.

Product Features

	Size determination of dsDNA on agarose gel
	Formulated with loading dye for direct loading on gel
	Stable at room temperature
	Reference band for orientation

Product Specifications

Concentration
100 ng/µl

Storage and handling
Upon arrival, store kit components at room temperature or 4 °C for up to 6 months. Store at –20 °C for longer-term storage.

Storage buffer

0.017% SDS, 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, 0.021% Orange G, 0.004% xylene cyanol and 10% glycerol.

DNA loading dye (6X)
0.1% SDS, 60 mM Tris-HCl (pH 8.0), 60 mM EDTA, 0.125% Orange G, 0.025% xylene cyanol and 60% glycerol.

Quality control
The absence of nucleases is confirmed by agarose gel electrophoresis showing well defined bands and no degradation.

Product Use Limitations
Kit components are sold exclusively for research purposes and in vitro use. Neither the product, nor any individual components, were tested for use in diagnostic applications or for drug development, nor is it suitable for administration to humans or animals. Please refer to the MSDS, available upon request.

Loading Protocol
1. If stored at -20 °C, mix gently after thawing

2. Load 5 µl per 1 mm gel lane

Note

	Do not heat before loading
	Dilute DNA sample with the DNA Loading Dye (6X): mix 1 volume of the dye solution with 5 volumes of the DNA sample
	For quantification, load an amount of sample similar to the nearest band of the KAPA Express Ladder
	Visualize DNA by staining with ethidium bromide or with SYBR® Green I dye

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