Products >> Lifescience >> Molecular Biology Reagents >> Kapa Taq
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Kapa Taq

Product Description

KapaTaq is the single-subunit Taq DNA polymerase enzyme from the thermophilic bacterium Thermus aquaticus, purified from recombinant Escherichia coli. Taq polymerizes DNA from a primer annealed to a DNA template in the presence of deoxyribonucleoside triphosphates. Taq possesses 5’g3’ polymerase activity, as well as double-strand dependent 5’g3’ exonuclease activity. The enzyme lacks 3’g5’ exonuclease activity and therefore does not possess a proofreading function.

All KapaTaq kits are supplied with high yield and standard reaction buffers and separate magnesium chloride solution to accommodate PCR
optimization.

Product Applications

 Standard PCR
DNA labelling
DNA sequencing
Numerous applications for which a high-quality, thermostable DNA polymerase is required


Product Performance

HIGH YIELD AND SENSITIVITY

PCR reactions were performed on a 700 bp plasmid target using 1 unit of Taq polymerase in standard reaction buffer as recommended by each supplier. Sensitivity of the reaction was tested using a 10x template dilution series starting at 1 ng of DNA. As can be seen from the results, KapaTaq exhibits greater sensitivity as compared with competing suppliers.

Quality Control

KapaTaq is extensively purified to >99% total protein and is free of contaminating exonuclease and endonuclease. KapaTaq meets strict requirements with regard to DNA contamination.
KapaTaq is ideal for PCR reactions where endogenous templates would lead to misleading amplification products.

Kapa Taq with Loading Dye

KapaTaq DNA polymerase is also available with loading/tracking dye reaction buffer. The green loading dye will not inhibit the efficiency of the PCR reaction (Fig. 1) allowing you to save time and effort without compromising performance.

After PCR cycling you can simply load your PCR product directly onto the agarose gel with no extra steps for adding loading or tracking dye (Fig. 2).

Figure 1. KapaTaq with Loading Dye Reaction Buffer (B) shows no inhibition of PCR performance when compared to KapaTaq Reaction Buffer without tracking dye (A). Sensitivity was tested on a 10X dilution series starting with 1 ng of human genomic DNA and Alu primer set

Figure 2. KapaTaq reactions with 1X Loading Dye Reaction Buffer A. Volumes above wells indicate the volume of the PCR reaction loaded on the gel.

B. On a 1% agarose gel, the blue dye migrates at the same rate as a 5 kb DNA fragment, and the yellow dye migrates at 75 bp.

Kapa Taq Ready Mix

KapaTaq is also avaliable in a 2X ReadyMix format that contains KapaTaq DNA Polymerase, reaction buffer MgCl2, and dNTPs. Just add primers, template & PCR-grade water.

Kapa Taq Kit Components :

KapaTaq DNA Polymerase (5 U/ul in storage buffer)

High Yield Reaction Buffer with Mg2+ (Buffer A)
10x Standard Reaction Buffer with Mg2+ (Buffer B)
25 mM MgCl2

 

Kapa Taq
Product Code Kit Size
KK1014 250 units
KK1015 500 units
BK1000 2500 units
BK1002 5000 units

Kapa Taq Ready Mix
Product Code Kit Size
KK1006 250 rxn

Kapa Taq with loading dye
Product Code Kit Size
KK1020 250 units
KK1022 500 units
BK1004 2500 units
BK1006 5000 units

Kapa Taq Ready Mixwith loading dye
Product Code Kit Size
KK1024 250 rxn

* All kits are also available with Kapa dNTPs

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