Products >> Lifescience >> Molecular Biology Reagents >> Kapa Long Range Taq
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Kapa Long Range Taq

The KAPALong Range PCR system is a blend of Taq DNA polymerase and modified archaeal (Tpe B) DNA Polymerase processing proofreading capability. This two enzyme system is designed specifically to support long range and/or sensitive PCR. The KAPALongRange system polymerizes DNA from a primer annealed to a DNA templae in the presence of deoxyribonucleotide triphosphates. Both enzymes possed 5'->3' polymerase activily, but only Taq possesses double strand dependent 5'->3' exonuclease activity and only the Type B Polymerase possesses 3'->5' exonuclease (proofreading) activity.

KAPALongReach possesses higher fidelity than Taq Polymerase.

The majority of PCR products from a KAPALongRange DNA Polymerase reaction have a single deoxyadenosine added to the 3' ends of the amplicon and can be used for ligation into single 3' T overhang cloning sites.

All KAPALongRange kits are supplied with a 5x reaction buffer. This buffer  has been formulated to support long range and low template applications. Magnesium chloride solution is supplied separately to accommodate PCR optimization.

Product Application

	PCR amplification of long targets and/or PCR using low amounts of template DNA
	Standard short and mid-range PCR amplification
	Production of PCR products to be used for ligation into 3'_T_overhang cloning vectors

Product Performance

Long Range and High Sensitivity

KAPALongRange is able to amplify long templates from limiting complex genomic DNA.

Figure 1 : Amplification of 15 kb fragment (tPA gene) from human chromosomal DNA. Lanes:(M) Marker, (1) 25 ng human gDNA, (2) 5 ng human gDNA, (3) 1 ng human gDNA. 35 cycles, 68 degree c extension temperature, 2.5 units of enzyme.

KAPALongRange exhibits high yield, greater sensitivity and specificity compared with leading competitive long range kits.

Figure 2 : Amplification of 15 kb fragment from 10 pg, 10 fg, and 1 fg of lambda DNA. Lanes:(13) KAPALongRange, (M) Marker, (4-6) Competitor R, (7-9) Competitor T.
 

Product Performance

KAPALongRange can replace Taq polymerase using standard PCR cycling conditions and less enzyme per reaction. KAPALongRange amplifies short range and mid range targets with greater sensitivity and specificity as compared with Taq polymerase.

Figure 3: Amplification of 6 log dilution series of lambda DNA starting with 1 ng to 10 fg. Amplicons were 2kb, 8kb and 15kb in length. 35 cycles, 72 degree c extension temperature, 1.25 units of enzyme.

KAPALongRange kit components

	KAPALongRange DNA polymerase (5U/µl in storage buffer)
	5x KAPALongRange reaction buffer
	25 mM MgCL2 solution
	250µl KAPA dNTP Mix (10mM reach)

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