Real Time Master Mix
Kapa Biosystems offers a portfolio of
next-generation reagents that contain novel DNA
polymerases engineered specifically for your PCR
application. These enzymes are fundamentally
different than wild-type polymerases at the protein
level and contain unique amino acid modifications
that confer dramatic improvements to the function
and performance of the enzyme.
The performance benefits of these products enable
you to perform research and obtain results never
possible before. Our high performance reagents are
available for applications such as real-time PCR,
high fidelity PCR, next-generation DNA sequencing,
fast PCR, hot start PCR, crude sample PCR, robust
PCR, and molecular diagnostics.
KAPA PROBE FAST qPCR Kits
Precise, reproducible, and versatile for all
probe-based qPCR applications.
KAPA PROBE FAST qPCR Kits contain a ready-to-use
master mix for highly sensitive and accurate
real-time PCR using sequence-specific fluorogenic
probe chemistries including hydrolysis probes (e.g.
TaqMan®), FRET probes, and displacement probes (e.g.
molecular beacons).
Optimized for versatility and speed - KAPA PROBE
FAST qPCR Kits provide fast and reproducible results
for genotyping, gene expression analysis, and
multiplexing offering:
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KAPA SYBR FAST qPCR Kits
Containing the first DNA polymerase evolved for
real-time PCR.
KAPA SYBR® FAST qPCR Kits are designed for the highest
performance in real-time PCR. Kits contain a novel DNA
polymerase engineered through a process of molecular
evolution - the result is a unique enzyme, specifically
evolved for qPCR using SYBR® Green I dye chemistry.
The KAPA SYBR® DNA Polymerase was engineered to perform
optimally in stringent real-time qPCR reaction
conditions, exhibiting dramatic improvements to
signal-to-noise ratio (fluorescence), cycle threshold
(Ct), linearity, and sensitivity. The KAPA SYBR® DNA
Polymerase and proprietary buffer system enhances the
amplification efficiency of difficult templates,
including both GC-rich and AT-rich templates.
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KAPA2G Robust PCR Kits
Streamline your PCR workflows by consolidating protocols
and reaction conditions, increase pass rates, amplify
difficult templates without bias, and increase
sensitivity – all with a single enzyme.
The second-generation KAPA2G Robust DNA Polymerase was
evolved to solve - achieve consistent amplification
across a broad range of amplicon types (both GC and
AT-rich). The versatility and robustness of the
polymerase allows for consolidation of PCR cycling
protocols and reaction conditions while increasing
success rates. The high performance of the KAPA2G Robust
DNA Polymerase eliminates the need for multiple enzymes
and protocols - standardize your PCRs with a single
enzyme solution.
The KAPA2G Robust DNA Polymerase offers :
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Robust performance across a wide range of
GC- and AT-rich templates |
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Increased PCR success rates |
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Consolidated PCR protocols and reaction
conditions with a single enzyme Improved
tolerance to common PCR inhibitors for crude
samples and/or DNA extractions |
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Consistent Colony PCR from E. coli and yeast |
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Higher yield and sensitivity per unit of
enzyme |
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Three novel buffers and a proprietary PCR
enhancer offer extended optimization options
for the most difficult templates KAPA2G
Robust DNA Polymerase is also available with
HotStart technology for improved specificity
during prolonged benchtop setup or liquid
handling. |
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KAPA HiFi PCR Kits
A
second-generation polymerase engineered for extreme
fidelity and robustness - 100x fidelity improvement
compared to Taq polymerase.
KAPA HiFi DNA Polymerase is a novel, single-enzyme
system that exhibits industry-leading performance
when compared with other high fidelity polymerases
and polymerase blends. KAPA HiFi has been engineered
to have an increased affinity for DNA without the
need for accessory protein domains. The intrinsic
high processivity of KAPA HiFi DNA Polymerase
results in significant improvements to yield,
sensitivity, speed, target length, and the ability
to amplify difficult templates (e.g GC-rich) -
standardize your high fidelity PCRs with a single
enzyme.
The engineered KAPA HiFi DNA Polymerase plus
optimized buffer system offers:
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Superior fidelity - 100x improvement
compared to Taq polymerase. |
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Robust performance across a wide range of
difficult templates, including both AT- and
GC-rich amplicons. |
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Consolidation of PCR protocols and reaction
conditions with single enzyme. |
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Long range amplification of complex targets
- up to 15 kb from genomic DNA. |
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High speed - reduce reaction times by up to
75%. |
KAPA HiFi DNA Polymerase is also available with
HotStart technology for improved specificity
during prolonged benchtop setup or liquid
handling.
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KAPA2G Fast PCR Kits
A second-generation DNA polymerase engineered
for the ultimate in speed and performance.
KAPA2G Fast DNA Polymerase is a
second-generation enzyme derived through a
process of molecular evolution. The KAPA2G Fast
DNA Polymerase was engineered for higher
processivity and speed, offering significantly
faster extension rates and improved PCR success
rates than wild-type Taq polymerase. KAPA2G Fast
is well suited for both routine and
high-throughput PCR, achieving higher yields and
improved sensitivity than competitor enzymes
across a wide range of amplicon types.
KAPA2G Fast DNA Polymerase and optimized buffer
system offers:
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Extension times as low as 1 sec per kilobase. |
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Outstanding performance when compared to
standard and hot start Taq polymerase. |
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20 - 70% reductions in total PCR time. |
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No requirement for specialist PCR
consumables or instrumentation. |
KAPA2G Fast DNA Polymerase is also available
with HotStart technology for improved
specificity during prolonged benchtop setup or
liquid handling.
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KAPA Long Range PCR Kits
Amplify
long templates up to 20 kb with extreme
sensitivity and increased fidelity.
The KAPA Long Range PCR system is a blend of Taq
DNA polymerase and an engineered archaeal
(B-family) DNA polymerase possessing
proofreading capability. This two-enzyme system
is designed specifically to support long range
and/or sensitive PCR.
KAPA Long Range DNA Polymerase possesses 3x – 4x
higher fidelity than Taq polymerase. The
majority of PCR products from a KAPA Long Range
HotStart DNA Polymerase reaction have a single
deoxyadenosine added to the 3’ ends of the
amplicon and can be used for ligation into
single 3’-T -overhang cloning sites.
The KAPA Long Range PCR system is ideally suited
for:
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PCR amplification of long targets up to 20
kb and/or PCR using low amounts of template
DNA. |
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Standard short- and mid-range PCR
amplification. |
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Production of PCR products to be used for
ligation into 3’-T-overhang cloning vectors. |
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Targeted sample enrichment for
next-generation DNA sequencing. |
KAPA Long Range DNA Polymerase is also available
with HotStart technology for improved
specificity during prolonged benchtop setup or
liquid handling.
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KAPA Blood PCR Kits
Eliminate the need for DNA extraction with the
first DNA polymerase evolved for whole blood PCR.
KAPA Blood DNA Polymerase is a second-generation
enzyme derived through a process of molecular
evolution and the first DNA polymerase
engineered specifically for the amplification of
DNA directly from whole blood. The enzyme is
available in two optimized, easy-to-use 2x PCR
mixes, which contain all components required for
Whole Blood PCR, except primers and template
(blood). Using KAPA Blood PCR Kits, DNA
fragments may be amplified directly from
reactions containing 1 - 20% (v/v) whole human
blood without pretreatment of blood samples or
DNA isolation, significantly reducing
contamination risk, turnaround time and cost of
genetic testing.
KAPA Blood PCR kits offer:
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Direct amplification from fresh or frozen
whole blood, blood collected in EDTA tubes
or on FTA® Elute Cards, “Guthrie cards” or
filter paper. |
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Eliminates the need for DNA extraction in
genetic testing protocols for humans, other
mammals and birds. |
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Reduces contamination risk, turnaround time
and cost of genetic testing. |
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Compatible with existing workflows, standard
protocols and routinely used detection
methods. |
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KAPA Taq PCR Kits
KAPA
Taq DNA Polymerase is based on the
single-subunit, wild-type Taq DNA polymerase of
the thermophilic bacterium Thermus aquaticus. In
the HotStart formulation, the enzyme is combined
with a proprietary antibody that inactivates the
enzyme until the first denaturation step. This
eliminates spurious amplification products
resulting from non-specific priming events
during reaction setup and initiation, and
increases overall reaction efficiency and
sensitivity.
KAPA Taq and KAPA Taq HotStart DNA Polymerase is
ideally suited for:
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High throughput PCR |
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Amplification of low copy DNA templates |
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Multiplex PCR |
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Specific amplification of complex templates |
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RT-PCR |
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